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Addition of benzyladenine to coconut explant cultured In vitro improves the formation of somatic embryos and their germination Agrociencia
Montero-Cortés,Mayra I.; Chan-Rodríguez,José L.; Cordova-Lara,Ivan; Oropeza-Salin,Carlos; Sáenz-Carbonell,Luis.
Coconut (Cocos nucífera L.) production has been declining in México and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocos nucífera; Clonal propagation; Somatic embryogenesis; Cytokinin.
Ano: 2011 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000600002
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Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae) Genet. Mol. Biol.
Oliveira,Luiz Orlando de; Venturini,Bruna Afonso; Rossi,Ana Aparecida Bandini; Hastenreiter,Saulo Santos.
The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together to form clusters with well-defined borders. Cluster size may range from several to hundreds of aerial stems. To investigate the extent of clonality among aerial stems in ipecac clusters, we sampled 50 wild clusters (a total of 291 aerial stems) and screened them with 89 inter-simple sequence repeat (ISSR) markers. The 291 aerial stems were grouped into 42 putative clones. The clonal groups generally consisted of aerial stems from the same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clonal propagation; Genetic diversity; ISSR; Poaia; Psychotria ipecacuanha.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100016
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Sucrose concentration and volume of liquid medium on the in vitro growth and development of blackberry cv. Tupy in temporary immersion systems Ciência e Agrotecnologia
Ayub,Ricardo Antônio; Santos,Jessé Neves dos; Zanlorensi Junior,Luiz Antônio; Silva,Daurimar Mendes da; Carvalho,Tereza Cristina de; Grimaldi,Fernanda.
ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rubus spp.; Clonal propagation; Hyperhydricity..
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542019000100229
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Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus Ciência e Agrotecnologia
Alves,Marcos Nopper.
Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Clonal propagation; Adventitious buds; Growth regulators; Callogenesis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000500024
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